v5.0 [Oct 24, 2019]
New Functionality:
- Added tools for pairwise alignment of DNA and protein sequences.
- Added interfaces for simulating directional and blunt TOPO® cloning.
- Provided support for customizing the background window color.
- Added the ability to drag out and view non-aligned ends of aligned sequences.
- Added the ability to display feature translations in lowercase.
- Enabled alignments to be constrained to a designated strand or region of the reference DNA sequence.
- Added the ability to import sequences directly from Ensembl.
- Enabled the calculation and saving of codon frequencies for one or more translated features.
- Enabled feature visibility to be toggled by feature type.
- Added the option to trim low-quality ends when importing sequence traces for contig assembly or multiple sequence alignment.
- Enabled an aligned cDNA to be used to annotate a feature with exons and introns.
- Added support for opening DNASIS files.
- Enhanced BLAST support to provide all five options (blastn, blastx, tblastx, blastp, and tblastn).
- Updated the "Aligned Sequences" menu when viewing an alignment to a reference sequence, and added the following new commands: • Duplicate Selected Sequence(s) in New Window(s) • Remove All Sequences • Show/Hide Quality Data for Sequence Traces
- Added a control in Preferences to enable quality data for sequence traces to be shown by default when aligning to a reference DNA sequence.
- Enabled a selected portion of a multiple DNA alignment to be converted to a multiple protein alignment.
- Added a control for exporting selected files in a collection as a list in PDF or tab-separated format.
- Added a "Choose DNA Sequences..." button to the Simulate Agarose Gel dialog, as a shortcut for configuring multiple lanes.
- Enabled amino acid sequences to be copied as either 1- or 3-letter amino acid codes.
- Enabled the copying of selections that span multiple lines in pairwise or multiple alignment windows.
- Added the New England Biolabs "TriDye™ Ultra Low Range DNA Ladder".
Enhancements:
- Calculated the total length of the segments for a feature with gaps, with this information now displayed in Features view, feature dialogs, and feature tooltips.
- Reduced the width of the "Code Number" column in the collection interface by using an icon.
- Added the option to preserve a feature translation by adjusting its reading frame when exiting the Edit Feature dialog.
- Added an option in Preferences to display the MW of a selection in a protein file in Daltons.
- Added a notification if some of the input sequences were not included when assembling contigs.
- Added a keyboard shortcut for importing primers from a list.
- Relaxed a restriction that prohibited characters in primer names.
- Improved the detection of T7 promoter features.
- Improved import from NCBI to tolerate regions in which the left and right endpoints are swapped, and to recognize "c" as denoting the reverse complement.
- Added support for new genetic codes.
- Improved the algorithm for aligning to a reference DNA sequence.
- Improved the import of features from BED and GTF files generated by geneXplain.
- Enhanced the Vector NTI® database importer to recognize separators.
- Provided the version numbers of alignment programs (e.g., MUSCLE) in the user interface.
- Improved the visibility of selections and their endpoints in maps by including lines that extend below the DNA line.
- Enhanced the "Edit MW Markers List" dialog to support display of MW markers by supplier, and to enable one-step selection of all MW markers from a supplier.
- Dramatically improved scrolling performance in Features view for sequences with many features.
- Added commands to the Enzymes and Primers menus for pulling up the corresponding tabs in Preferences.
- Enhanced Properties view in protein windows to list both average and monoisotopic molecular weights.
- Made the alignment dialogs modeless to enable switching to other SnapGene windows.
- Improved the visibility of a Consensus selection in a multiple alignment window by extending blue lines down from each end of the selection.
v4.2 [Jul 20, 2018]
- Increased visibility of the message about customizing the MW markers list for agarose gels.
- Fixed a regression with importing regions of sequences from NCBI.
(Reported by Susan Gilfillan)
- Improved application closing behavior on macOS 10.14.
- Improved general application stability.
v2.8 [Jul 4, 2015]
May include unspecified updates, enhancements, or bug fixes.
